Seri langsung RT-qPCR
PCR langsung
Apa iku Direct PCR
PCR langsung minangka cara amplifikasi DNA langsung saka getih, jaringan kewan, buntut tikus, sel, kuping tikus, iwak zebra, endhog iwak, godhong tanduran, wiji utawa sampel jaringan biologis asli liyane tanpa nindakake langkah-langkah isolasi lan pemurnian DNA.Teknik PCR langsung bisa nyuda wektu eksperimen, lan biaya ing genotipe lan proyek kanthi jumlah dhuwur.Iki uga menehi pilihan sing luwih apik nalika ngadhepi tantangan kanggo nggedhekake jumlah sampel sing cilik banget ing ngendi langkah pemurnian bisa nyebabake mundhut sampel.
Campuran PCR FOREGENE nduweni resistensi stres sing kuat, amarga kekuatan FOREGENE sing dipatenake enzim Taq (paten resmi: ZL20161034512.0 (China), EP3450560 (Eropa), PCT / CN2017/082154 (AS), paten 6894926 (Jepang)).Enzim Taq sawijining nggunakake teknologi rekombinasi DNA kanggo rekombinasi wilayah struktur naleni DNA saka protein naleni asam nukleat archaeal karo polimerase DNA Taq tahan panas kanggo mbangun DNA cithakan karo tambah afinitas.Taq wiwitan panas sing luwih apik sing nahan macem-macem fungsi polimerase DNA asli. Enzim Taq iki bisa ngiket DNA cithakan lan miwiti sintesis DNA ing cithakan konsentrasi rendah lan lingkungan kompleks.
Kayata, gampang kaya nambahake sel, tanduran (godhong enom, oyod utawa wiji) utawa conto kewan menyang buffer, lan nambah campuran master unik karo primer khusus ing tabung PCR lan mlaku liwat siklus termal.Efisiensi kit cocog kanggo analisis sampel skala gedhe sing ngirit wektu penting banget.
Sistem reaksi PCR sing ngemot enzim UNG lan dUTP dipilih miturut syarat eksperimen, sing bisa nyegah polusi sing disebabake dening produk amplifikasi PCR.
Kaluwihan sakaDPCR langsung
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| PCR langsung | Cara tradisional (Pemurnian RNA + PCR Biasa) |
| Konsumsi bahan | Konsumsi jaringan | Kurang | akeh |
| Persiapan cithakan | Jinis materi | Ora mbutuhake grinding | Sampel penggilingan |
| Operasi | Tipe siji-tabung, 10 min | operasi rumit, 1h | |
| Fluks | 1-96 | 1-24 | |
| reaksi PCR | sistem reaksi | Optimized 2 × PCR campuran | Dntp, MgCl2,10×PCR Buffer, enzim Taq, |
Application
01 Identifikasi mikroorganisme patogen
02 Identifikasi modifikasi genetik, genotipe
03 Multiplex PCR / deteksi SNP / PCR-RFLP
04Sequencing/kloning
Seri produk
| Seri | Jeneng produk | Spesifikasi | Nomer Katalog | Kahanan Panyimpenan |
| Seri RT-qPCR langsung | 200T | DRT-01011 | Bagean I 4 ℃, Bagean II -20 ℃ | |
| 1000T | DRT-01012 |
| Seri | Jeneng produk | Spesifikasi | Nomer Katalog | Kahanan Panyimpenan |
|
Seri PCR Langsung Darah | 200 × 20μl rxns | TP-04111 | -20 ℃ | |
| 2000 × 20μl rxns | TP-04113 | |||
| 200 × 20μl rxns | TP-04121 | -20 ℃ | ||
| 2000 × 20μl rxns | TP-04123 | |||
| 200 × 20μl rxns | TP-04211 | -20 ℃ | ||
| 2000 × 20μl rxns | TP-04213 | |||
| 200 × 20μl rxns | TP-04221 | -20 ℃ | ||
| 2000 × 20μl rxns | TP-04223 |
| Series | Jeneng produk | Spesifikasi | Nomer Katalog | Kahanan Panyimpenan |
| Seri PCR Animal Direct | Kit PCR Langsung Tisu Kewan (65 ℃ lisis) | 200 × 20μl rxns | TP-01111 | Bagean I 4 ℃, Bagean II -20 ℃ |
| 2000 × 20μl rxns | TP-01113 | |||
| Kit PCR Langsung Tisu Kewan (65 ℃ lisis) -UNG | 200 × 20μl rxns | TP-01121 | Bagean I 4 ℃, Bagean II -20 ℃ | |
| 2000 × 20μl rxns | TP-01123 | |||
| Buntut Tikus SuperDirectTMPaket PCR (lisis suhu kamar) | 200 × 20μl rxns | TP-01311 | Bagean I 4 ℃, Bagean II -20 ℃ | |
| 2000 × 20μl rxns | TP-01313 | |||
| Kit PCR Langsung Buntut Tikus (65 ℃ lisis) | 200 × 20μl rxns | TP-01331 | Bagean I 4 ℃, Bagean II -20 ℃ | |
| 2000 × 20μl rxns | TP-01333 | |||
| Kit PCR Langsung Buntut Tikus (65 ℃ lisis) - UNG | 200 × 20μl rxns | TP-01341 | Bagean I 4 ℃, Bagean II -20 ℃ | |
| 2000 × 20μl rxns | TP-01343 | |||
| Kit PCR Langsung Ikan Zebra (65 ℃ lisis) | 200 × 20μl rxns | TP-01411 | Bagean I 4 ℃, Bagean II -20 ℃ | |
| 2000 × 20μl rxns | TP-01413 | |||
| Zebra Fish Direct PCR Kit(65 ℃ lysis) - UNG | 200 × 20μl rxns | TP-01421 | Bagean I 4 ℃, Bagean II -20 ℃ | |
| 2000 × 20μl rxns | TP-01423 |
| Seri | Jeneng produk | Spesifikasi | Nomer Katalog | Kahanan Panyimpenan |
| Pabrik Langsung PCR Series | 200 × 20μl rxns | TP-02111 | Bagean I 4 ℃, Bagean II -20 ℃ | |
| 2000 × 20μl rxns | TP-02113 | |||
| 200 × 20μl rxns | TP-02121 | Bagean I 4 ℃, Bagean II -20 ℃ | ||
| 2000 × 20μl rxns | TP-02123 | |||
| Tanduran Godhong Langsung PCR Plus Kit | 200 × 20μl rxns | TP-02131 | Bagean I 4 ℃, Bagean II -20 ℃ | |
| 2000 × 20μl rxns | TP-02133 | |||
| Tanduran Godhong Langsung PCR Plus Kit -UNG | 200 × 20μl rxns | TP-02141 | Bagean I 4 ℃, Bagean II -20 ℃ | |
| 2000 × 20μl rxns | TP-02143 | |||
| 200 × 20μl rxns | TP-03111 | Bagean I 4 ℃, Bagean II -20 ℃ | ||
| 2000 × 20μl rxns | TP-03113 | |||
| 200 × 20μl rxns | TP-03121 | Bagean I 4 ℃, Bagean II -20 ℃ | ||
| 2000 × 20μl rxns | TP-03123 | |||
| 200 × 20μl rxns | TP-03131 | Bagean I 4 ℃, Bagean II -20 ℃ | ||
| 2000 × 20μl rxns | TP-03133 | |||
| 200 × 20μl rxns | TP-03141 | Bagean I 4 ℃, Bagean II -20 ℃ | ||
| 2000 × 20μl rxns | TP-03143 | |||
| Bibit Tanaman Langsung PCR Plus Kit I (Polysaccharide polyphenol plant) | 200 × 20μl rxns | TP-03151 | Bagean I 4 ℃, Bagean II -20 ℃ | |
| 2000 × 20μl rxns | TP-03153 | |||
| Biji Tanaman Langsung PCR Plus Kit I - UNG(Polysaccharide polyphenol plant) | 200 × 20μl rxns | TP-03161 | Bagean I 4 ℃, Bagean II -20 ℃ | |
| 2000 × 20μl rxns | TP-03163 | |||
| 200 × 20μl rxns | TP-03171 | Bagean I 4 ℃, Bagean II -20 ℃ | ||
| 2000 × 20μl rxns | TP-03173 | |||
| Biji Tanaman Langsung PCR Plus Kit II - UNG(Polysaccharide polyphenol plant) | 200 × 20μl rxns | TP-03181 | Bagean I 4 ℃, Bagean II -20 ℃ | |
| 2000 × 20μl rxns | TP-03183 |
